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Code Name Applications Availability Size Tested species Price Grade  
EB05914 Goat Anti-TRIM2 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Rat $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05914 1
EB05914 (0.5µg/ml) staining of Rat Brain (A) + Peptide (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05914 2
EB05914 (6.3µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05914 3
EB05914 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM2, tripartite motif-containing 2, KIAA0517, tripartite motif protein 2, tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2
Official Symbol: TRIM2
Accession Number(s): NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1
Human GeneID(s): 23321
Non-Human GeneID(s): 80890 (mouse)
Important Comments: Cross-reactivity with TRIM3 has been demonstrated in Mouse.

Immunogen

Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75-85kDa band observed in Rat Brain lysates (calculated MW of 83.3 according to Rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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