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Goat Anti-EDD1 / HYD Antibody (EB05920)

Code Name Applications Availability Size Tested species Price Grade  
EB05920 Goat Anti-EDD1 / HYD Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB05920 1
EB05920 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05920 2
EB05920 (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBR5, EDD1, E3 ubiquitin protein ligase, HECT domain containing, 1, DD5, EDD, HYD, KIAA0896, progestin induced protein, ubiquitin-protein ligase, E3 identified by differential display, hyperplastic discs protein homolog, ubiquitin protein ligase E3 component n-recognin 5, FLJ11310, MGC57263
Official Symbol: UBR5
Accession Number(s): NP_056986.2
Human GeneID(s): 51366
Non-Human GeneID(s): 70790 (mouse), 117060 (rat)

Immunogen

Peptide with sequence C-LAIKTKNFGFV, from the C Terminus of the protein sequence according to NP_056986.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
IHC: In paraffin embedded Human Testis shows nuclear staining in all germ cells. Recommended concentration: 1-2µg/ml. Paraffin embedded Human Spleen. Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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