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Code Name Applications Availability Size Tested species Price Grade  
EB05932 Goat Anti-TRIM5 / RNF88 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05932 1
EB05932 (1.0µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.
EB05932 2
EB05932 (3µg/ml) staining of paraffin embedded Human Colon. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05932 3
EB05932 Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM5, RNF88, tripartite motif-containing 5, TRIM5alpha, tripartite motif protein 5, tripartite motif protein TRIM5, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Official Symbol: TRIM5
Accession Number(s): NP_149023.1; NP_149083.1; NP_149084.1
Human GeneID(s): 85363
Important Comments: This antibody is expected to recognise all three human isoforms of this protein.

Immunogen

Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1; NP_149083.1; NP_149084.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 55 and 37kDa bands observed in Human Thymus lysates (calculated MW of 56.3kDa according to NP_149023.1 and of 37.7kDa according to NP_149084.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Colon. Recommended concentration: 3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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