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Goat Anti-GNIP / TRIM7 Antibody (EB05937)

Related Products: EBP05937 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05937 Goat Anti-GNIP / TRIM7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05937 1
EB05937 (1µg/ml) staining of Human Skeletal Muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05937 2
EB05937 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM7, GNIP1, RNF90, tripartite motif-containing 7, tripartite motif protein TRIM7, glycogenin-interacting protein 1, GNIP, glycogenin-interacting protein
Official Symbol: TRIM7
Accession Number(s): NP_203128.1
Human GeneID(s): 81786

Immunogen

Peptide with sequence C-QAPAGPPWDITEA, from the C Terminus of the protein sequence according to NP_203128.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25-30kDa band observed in Human Skeletal Muscle lysate (predicted MW of 25kDa according to NP_203128). Recommended for use at 1µg/ml.
IHC: In paraffin embedded Human Skeletal Muscle shows pixulate staining in the fibres. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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