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Code Name Applications Availability Size Tested species Price Grade  
EB06000 Goat Anti-FOXA2 / HNF3B Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06000 1
EB06000 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06000 2
EB06000 (1µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
EB06000 3
EB06000 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXA2, HNF3B, forkhead box A2, TCF3B, MGC19807, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta, forkhead box protein A2, HNF-3B, HNF-3-beta, TCF-3B, transcription factor 3B
Official Symbol: FOXA2
Accession Number(s): NP_068556.2; NP_710141.1
Human GeneID(s): 3170
Non-Human GeneID(s): 15376 (mouse), 25099 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms: NP_068556.2; NP_710141.1

Immunogen

Peptide with sequence C-GVYSRPIMNSS, from the C Terminus of the protein sequence according to NP_068556.2; NP_710141.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 60kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and Lung for this particular batch.
IHC: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 4-8µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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