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Goat Anti-FOXB1 / FKH5 Antibody (EB06005)

Related Products: EBP06005 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06005 Goat Anti-FOXB1 / FKH5 Antibody Pep-ELISA, WB, ICC, IF Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06005 1
EB06005 (1µg/ml) staining of HepG2 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXB1, forkhead box B1, FKH5, HFKH-5
Official Symbol: FOXB1
Accession Number(s): NP_036314.2
Human GeneID(s): 27023
Non-Human GeneID(s): 64290 (mouse)

Immunogen

Peptide with sequence C-TSPASALHSVAVH, from the C Terminus of the protein sequence according to NP_036314.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 35.0kDa according to NP_036314.2. Recommended concentration: 1-3µg/ml.

Immunocytochemsitry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3ug/ml.
Immunofluorescence: This antibody has been successfully used in IF for mouse brain at embryonic day 13.5.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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