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Goat Anti-FOXE1 / TTF2 Antibody (EB06011)

Related Products: EBP06011 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06011 Goat Anti-FOXE1 / TTF2 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB06011 1
EB06011 (2µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
EB06011 2
EB06011 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXE1, TTF2, forkhead box E1 (thyroid transcription factor 2), FOXE2, HFKH4, HFKL5, TITF2, TTF-2, FKHL15, forkhead (Drosophila)-like 15, forkhead box E1, forkhead box E2, thyroid transcription factor-2, Forkhead, drosophila, homolog-like 15
Official Symbol: FOXE1
Accession Number(s): NP_004464.2
Human GeneID(s): 2304

Immunogen

Peptide with sequence C-AYPGGIDRFVSAM, from the C Terminus of the protein sequence according to NP_004464.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 70kDa band in Human Thymus and Thyroid Gland lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.1kDa according to NP_004464.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Thyroid Gland shows nuclear staining in thyrocytes. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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