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Goat Anti-CENTB1 / ACAP1 Antibody (EB06122)

Related Products: EBP06122 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06122 Goat Anti-CENTB1 / ACAP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB06122 1
EB056122 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACAP1, CENTB1, centaurin, beta 1, KIAA0050, KIAA0050 gene product, Arf GAP with coiled coil, ANK repeat and PH domains 1, ArfGAP with coiled-coil, ankyrin repeat and PH domains 1, centaurin, beta 1
Official Symbol: ACAP1
Accession Number(s): NP_055531.1
Human GeneID(s): 9744
Non-Human GeneID(s): 216859 (mouse)

Immunogen

Peptide with sequence C-EKLSRRSHDLHTL, from the C Terminus of the protein sequence according to NP_055531.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in Jurkat lysates (calculated MW of 81.5kDa according to NP_055531.1). Recommended for use at 0.3-1µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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