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Goat Anti-CENTB2 / ACAP2 Antibody (EB06123)

Related Products: EBP06123 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06123 Goat Anti-CENTB2 / ACAP2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06123 1
EB06123 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06123 2
EB06123 (0.3µg/ml) staining of Mouse (A) and Rat (B) Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06123 3
EB06123 (0.3µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06123 4
EB06123 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACAP2, CENTB2, centaurin, beta 2, CNT-B2, KIAA0041, centaurin beta2, Arf GAP with coiled coil, ANK repeat and PH domains 2, ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Official Symbol: ACAP2
Accession Number(s): NP_036419.3
Human GeneID(s): 23527
Non-Human GeneID(s): 78618 (mouse)

Immunogen

Peptide with sequence C-EKLNRFQQDSQKF, from the C Terminus of the protein sequence according to NP_036419.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Mouse and Rat Lung lysates and in lysates of cell lines MOLT4 and NIH3T3 (calculated MW of 88.1kDa according to Human NP_036419 and 87.2kDa according to Mouse NP_084414.1 and Rat NP_001029178.1). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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