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Goat Anti-Cystatin B / Stefin B Antibody (EB06131)

Related Products: EBP06131 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06131 Goat Anti-Cystatin B / Stefin B Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06131 1
EB06131 (0.1µg/ml) staining of Human Lung Lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06131 2
EB06131 (0.1µg/ml) staining of Human Lung Lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06131 3
EB06131 (7µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06131 4
EB06131 (7µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06131 5
EB06131 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
EB06131 6
EB06131 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CSTB, cystatin B (stefin B), PME, CST6, STFB, CPI-B, stefin B, liver thiol proteinase inhibitor, EPM1, cystatin B, CSTB, cystatin B (stefin B), PME, CST6, STFB, CPI-B, stefin B, liver thiol proteinase inhibitor, EPM1, cystatin B
Official Symbol: CSTB
Accession Number(s): NP_000091.1
Human GeneID(s): 1476

Immunogen

Peptide with sequence C-QTNKAKHDELTYF, from the C Terminus of the protein sequence according to NP_000091.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 16kDa band observed in Human Liver, Lung and Kidney Lysates (calculated MW of 11.1kDa according to NP_000091.1 ). The observed molecular weight corresponds to earlier findings in literature with with different antibodies (Lee et al, Clin Cancer Res. 2008 Feb 15;14(4):1080-9.; PMID: 18281540 ). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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