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Goat Anti-ORP3 Antibody (EB06153)

Related Products: EBP06153 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06153 Goat Anti-ORP3 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06153 1
EB06153 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06153 2
EB06153 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: OSBPL3, ORP3, ORP-3, OSBP3, KIAA0704, MGC21526, oxysterol binding protein-like 3, OSBP-related protein 3, oxysterol-binding protein 3, oxysterol-binding protein-related protein 3, DKFZp667P1518, OTTHUMP00000122490, OTTHUMP00000122491, OTTHUMP00000202019, oxysterol-binding protein-like protein 3
Official Symbol: OSBPL3
Accession Number(s): NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1
Human GeneID(s): 26031
Non-Human GeneID(s): 71720 (mouse)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1).

Immunogen

Peptide with sequence MSDEKNLGVSQKL-C, from the N Terminus of the protein sequence according to NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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