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Goat Anti-CYP7B1 Antibody (EB06161)

Related Products: EBP06161 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06161 Goat Anti-CYP7B1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06161 1
EB06161 (4µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CYP7B1, CP7B, cytochrome P450, family 7, subfamily B, polypeptide 1, oxysterol 7alpha-hydroxylase, cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1, CBAS3, SPG5A
Official Symbol: CYP7B1
Accession Number(s): NP_004811.1
Human GeneID(s): 9420

Immunogen

Peptide with sequence C-YPDSDVLFRYKVKS, from the C Terminus of the protein sequence according to NP_004811.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Breast shows textured cytoplasm staining in ductal epithelial cells. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested:
Expected from sequence similarity: Human, Cow

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