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Goat Anti-P114-RHO-GEF / ARHGEF18 Antibody (EB06163)

Related Products: EBP06163 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06163 Goat Anti-P114-RHO-GEF / ARHGEF18 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB06163 1
EB06163 (0.5µg/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18
Official Symbol: ARHGEF18
Accession Number(s): NP_056133.2; NP_001124427.1
Human GeneID(s): 23370
Important Comments: This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).

Immunogen

Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 36404918.

Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in WB and IF on Human:
Reiko Satow, Yudai Suzuki, Shinobu Asada, Sae Ota, Masashi Idogawa, Shiori Kubota, Noi Ikeo, Atsuko Yoneda, Kiyoko Fukami
Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells.
iScience. 2022 Nov 4;25(12):105501.
PMID: 36404918

This antibody (previous batch) has been successfully used in WB on Medaka:
Herder C, Swiercz JM, Müller C, Peravali R, Quiring R, Offermanns S, Wittbrodt J, Loosli F.
ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation.
Development. 2013 Jul;140(13):2787-97.
PMID: 23698346

This antibody (previous batch) has been successfully used in WB on Human:
Terry SJ, Elbediwy A, Zihni C, Harris AR, Bailly M, Charras GT, Balda MS, Matter K.
Stimulation of Cortical Myosin Phosphorylation by p114RhoGEF Drives Cell Migration and Tumor Cell Invasion.
PLoS One. 2012;7(11):e50188.
PMID: 23185572

This antibody (previous batch) has been successfully used in WB, IF and IP on Human:
Terry SJ, Zihni C, Elbediwy A, Vitiello E, Leefa Chong San IV, Balda MS, Matter K.
Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis.
Nat Cell Biol. 2011 Feb;13(2):159-66.
PMID: 21258369

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