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Goat Anti-Pleckstrin Antibody (EB06169)

Related Products: EBP06169 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06169 Goat Anti-Pleckstrin Antibody Pep-ELISA, WB, IF, FC, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06169 1
EB06169 staining (0.01µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06169 2
EB06169 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06169 3
EB06169 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06169 4
EB06169 (3.75µg/ml) staining of paraffin embedded Human Vessel (platelets). Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
EB06169 5
EB06169 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PLEK, pleckstrin, P47, p47, FLJ27168
Official Symbol: PLEK
Accession Number(s): NP_002655.2
Human GeneID(s): 5341

Immunogen

Peptide with sequence C-EWIKAIQMASRTGK, from the C Terminus of the protein sequence according to NP_002655.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40-45kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1kDa according to Human NP_002655.2). Recommended concentration: 0.01-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 ( hepatocellular carcinoma) cell lysate.
IHC: Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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