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Goat Anti-KPNA1 / Importin alpha 5 Antibody (EB06232)

Code Name Applications Availability Size Tested species Price Grade  
EB06232 Goat Anti-KPNA1 / Importin alpha 5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB06232 1
EB06232 staining (1µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: KPNA1, importin alpha 5, RCH2, SRP1, NPI-1, karyopherin alpha 1 (importin alpha 5), IPOA5, importin-alpha-S1, karyopherin alpha 1, nucleoprotein interactor 1, recombination activating gene cohort 2
Official Symbol: KPNA1
Accession Number(s): NP_002255.3
Human GeneID(s): 3836
Non-Human GeneID(s): 16646 (mouse), 288064 (rat)

Immunogen

Peptide with sequence TTPGKENFRLKSY-C, from the N Terminus of the protein sequence according to NP_002255.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 60.3kDa according to NP_002255.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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