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Goat Anti-RANGAP1 Antibody (EB06253)

Related Products: EBP06253 - Immunizing Peptide, Goat Anti-RANGAP1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06253 Goat Anti-RANGAP1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06253 1
EB06253 staining (0.2µg/ml) of 3T3 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB06253 2
EB06253 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RANGAP1, KIAA1835, Ran GTPase activating protein 1, Fug1, MGC20266, SD, OTTHUMP00000198756, OTTHUMP00000198757, segregation distorter homolog, segregation distortion
Official Symbol: RANGAP1
Accession Number(s): NP_002874.1
Human GeneID(s): 5905
Non-Human GeneID(s): 19387 (mouse)
Important Comments: Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.

Immunogen

Peptide with sequence ASEDIAKLAETLAK-C, from the N Terminus of the protein sequence according to NP_002874.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5µg/ml.
IHC: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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