Change country
Home

Goat Anti-SPA1 / SIPA1 Antibody (EB06260)

Related Products: EBP06260 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06260 Goat Anti-SPA1 / SIPA1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06260 1
EB06260 staining (1µg/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SIPA1, SPA1, MGC17037, signal-induced proliferation-associated gene 1, p130 SPA-1, GTPase-activating protein Spa-1, signal-induced proliferation-associated 1, MGC102688, signal-induced proliferation-associated gene 1, signal-induced proliferation-associated protein 1
Official Symbol: SIPA1
Accession Number(s): NP_694985.28; NP_006738.3
Human GeneID(s): 6494
Important Comments: Both variants represent identical protein (NP_694985.28 and NP_006738.3).

Immunogen

Peptide with sequence C-ASKQLGSPTADLA, from the C Terminus of the protein sequence according to NP_694985.28; NP_006738.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3µg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Product Reviews

Please login to review this product.