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Goat Anti-Dispatched homolog 1 Antibody (EB06299)

Related Products: EBP06299 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06299 Goat Anti-Dispatched homolog 1 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06299 1
EB06299 (2.5ug/ml) staining of U87. Detected by immunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.
EB06299 2
EB06299 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DISPA, MGC13130, MGC16796, DKFZP434I0428, dispatched A, DISP1, dispatched homolog 1, dispatched homolog 1 (Drosophila), FLJ43740, MGC104180
Official Symbol: DISP1
Accession Number(s): NP_116279.2; NP_001337559.1
Human GeneID(s): 84976

Immunogen

Peptide with sequence C-SELSGESLLIKTL, from the C Terminus of the protein sequence according to NP_116279.2; NP_001337559.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in the cell membrane of U87 cells. Recommended concentration: 2.5ug/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

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