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Goat Anti-ARPC3 Antibody (EB06354)

Related Products: EBP06354 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06354 Goat Anti-ARPC3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06354 1
EB06354 staining (1µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARPC3, ARC21, p21-Arc, actin related protein 2/3 complex, subunit 3, 21kDa, ARP2/3 protein complex subunit p21, actin related protein 2/3 complex subunit 3
Official Symbol: ARPC3
Accession Number(s): NP_005710.1
Human GeneID(s): 10094
Non-Human GeneID(s): 56378 (mouse)
Important Comments: Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).

Immunogen

Peptide with sequence C-RQFMNKSLSGPGQ, from the C Terminus of the protein sequence according to NP_005710.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Band observed just below 20kDa marker in HeLa, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3µg/ml. Primary incubation was for 1 hour. This antibody has been successfully used in Western blot on Mouse: Fu X et al (2013). PMID: 23303949.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Fu X, Brown KJ, Yap CC, Winckler B, Jaiswal JK, Liu JS.
Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons.
Neurosci. 2013 Jan 9;33(2):709-21.
PMID: 23303949

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