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Goat Anti-FOXO3A / FOXO3 / FKHRL1 Antibody (EB06392)

Related Products: EBP06392 - Immunizing Peptide, Goat Anti-FOXO3A / FOXO3 / FKHRL1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06392 Goat Anti-FOXO3A / FOXO3 / FKHRL1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06392 1
EB06392 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Detected by chemiluminescence.
EB06392 2
EB06392 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06392 3
EB06392 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, FOXO3, forkhead box O3, FOXO2, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
Official Symbol: FOXO3
Accession Number(s): NP_001446.1
Human GeneID(s): 2309
Non-Human GeneID(s): 56484 (mouse)
Important Comments: Both variants represent identical protein (NP_001446.1 and NP_963853.1).

Immunogen

Peptide with sequence C-GAKQASSQSWVPG, from the C Terminus of the protein sequence according to NP_001446.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 70kDa band observed in Human Heart lysates (calculated MW of 71.3kDa according to NP_001446.1). Recommended concentration: 0.1-1 µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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