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Goat Anti-CD2BP2 (N Terminus) Antibody (EB06414)

Related Products: EBP06414 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06414 Goat Anti-CD2BP2 (N Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
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EB06414 1
EB06414 (0.3µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06414 2
EB06414 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD2BP2, FWP010, LIN1, Snu40, CD2 antigen (cytoplasmic tail) binding protein 2, CD2 (cytoplasmic tail) binding protein 2, CD2 antigen (cytoplasmic tail)-binding protein 2, U5-52K, CD2 binding protein 2, CD2 cytoplasmic domain binding protein 2, U5 snRNP 52K protein
Official Symbol: CD2BP2
Accession Number(s): NP_006101.1
Human GeneID(s): 10421

Immunogen

Peptide with sequence PKRKVTFQGVGDE-C, from the N Terminus of the protein sequence according to NP_006101.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in Human Lymph Node, Thymus and Tonsil lysates (calculated MW of 37.6kDa according to NP_006101.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nishizawa et al, Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14897-902). Recommended concentration: 0.3-1.0µg/ml.
IHC: In paraffin embedded Human Spleen shows nucleolar staining in select splenocytes. Recommended concentration: 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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