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Code Name Applications Availability Size Tested species Price Grade  
EB06419 Goat Anti-RAN Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06419 1
EB06419 (0.5µg/ml) staining of A431 (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06419 2
EB06419 (3µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06419 3
EB06419 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: RAN, TC4, ARA24, RAN, member RAS oncogene family, Gsp1, OK/SW-cl.81, RanGTPase, guanosine triphosphatase Ran, member RAS oncogene family, ras-related nuclear protein
Official Symbol: RAN
Accession Number(s): NP_006316.1
Human GeneID(s): 5901
Non-Human GeneID(s): 19384 (mouse), 84509 (rat)

Immunogen

Peptide with sequence AAQGEPQVQFKLV-C, from the N Terminus of the protein sequence according to NP_006316.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25kDa band observed in lysates of cell lines A431, HeLa, HepG2 and NIH3T3 (calculated MW of 24.4kDa according to NP_006316.1). Recommended for use at 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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