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Goat Anti-XAGE1 (Isoform 1d) / GAGED2 Antibody (EB06434)

Related Products: EBP06434 - Immunizing Peptide, EBP06435 - Immunizing Peptide, Goat Anti-XAGE1 (Isoforms 1a, 1b and 1c) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06434 Goat Anti-XAGE1 (Isoform 1d) / GAGED2 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06434 1
EB06434 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GAGED2, XAGE1, XAGE-1, G antigen, family D, 2, XAGE-1 protein
Official Symbol: GAGED2
Accession Number(s): NP_597673.1
Human GeneID(s): 9503
Important Comments: This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).

Immunogen

Peptide with sequence CGFGFRRQGEDNT, from the C Terminus of the protein sequence according to NP_597673.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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