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Goat Anti-VAV3 Antibody (EB06467)

Related Products: EBP05250 - Immunizing Peptide, EBP06467 - Immunizing Peptide, Goat Anti-VAV3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06467 Goat Anti-VAV3 Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06467 1
EB06467 (7.5µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06467 2
EB06467 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
EB06467 3
EB06467 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06467 4
EB06467 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: VAV3, vav 3 oncogene, vav 3 guanine nucleotide exchange factor, FLJ40431
Official Symbol: VAV3
Accession Number(s): NP_006104.4; NP_001073343.1
Human GeneID(s): 10451
Important Comments: This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).

Immunogen

Peptide with sequence C-SGEQGTLKLPEK, from the internal region of the protein sequence according to NP_006104.4; NP_001073343.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 7.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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