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Goat Anti-FHL3 / SLIM2 Antibody (EB06509)

Code Name Applications Availability Size Tested species Price Grade  
EB06509 Goat Anti-FHL3 / SLIM2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB06509 1
EB06509 (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FHL3, SLIM2, four and a half LIM domains 3, LIM-only protein FHL3, MGC19547, MGC23614, MGC8696
Official Symbol: FHL3
Accession Number(s): NP_004459.2
Human GeneID(s): 2275
Non-Human GeneID(s): 56726 (mouse), 302363 (rat)

Immunogen

Peptide with sequence DQVLCQGCSQAGP, from the C Terminus of the protein sequence according to NP_004459.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 30kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 31.2kDa according to NP_004459.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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