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Goat Anti-RXR beta Antibody (EB06524)

Related Products: EBP06524 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06524 Goat Anti-RXR beta Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06524 1
EB06524 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06524 2
HEK293 overexpressing RXRB (RC200516) and probed with EB06524 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: RXRB, NR2B2, DAUDI6, RCoR-1, MGC1831, H-2RIIBP, retinoid X receptor, beta, MHC class I promoter binding protein, DADB-100D22.1
Official Symbol: RXRB
Accession Number(s): NP_068811.1
Human GeneID(s): 6257
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 70-83 of human RXR beta protein and does not cross-react with either RXR alpha or gamma.

Immunogen

Peptide with sequence CRDGMGDSGRDSRSP, from the internal region of the protein sequence according to NP_068811.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in lysates of cell line A431 (calculated MW of 56.9kDa according to NP_068811.1). In transfected HEK293 transiently expressing RXRB a band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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