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Goat Anti-ABAD / HADH2 Antibody (EB06598)

Related Products: EBP06598 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06598 Goat Anti-ABAD / HADH2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06598 1
EB06598 staining (0.3µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase
Official Symbol: HSD17B10
Accession Number(s): NP_004484.1; NP_001032900.1
Human GeneID(s): 3028
Important Comments: This antibody is expected to recognise both reported isoforms.

Immunogen

Peptide with sequence CIRLDGAIRMQP, from the C Terminus of the protein sequence according to NP_004484.1; NP_001032900.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature. A minor band of unknown identity was also consistently observed at appr 38kDa. This band was successfully blocked by incubation with the immunising peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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