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Goat Anti-GSTM1 / GSTM2 Antibody (EB06606)

Related Products: EBP06606 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06606 Goat Anti-GSTM1 / GSTM2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB06606 1
EB06606 (0.3µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GSTM1, GSTM2, MU, H-B, GST1, GTH4, GTM1, MU-1, GSTM1-1, MGC26563, GSTM1a-1a, GSTM1b-1b, glutathione S-transferase M1, HB subunit 4, GST class-mu 1, glutathione S-aryltransferase, glutathione S-alkyltransferase, glutathione S-transferase, Mu-1, glutathione S-aralkyltransferase, S-(hydroxyalkyl)glutathione lyase, glutathione S-transferase mu 1, glutathione S-transferase mu 2 (muscle), GST4, GSTM, GSTM2-2, GTHMUS, MGC117303, GST class-mu 2, GST, muscle, S-(hydroxyalkyl)glutathione lyase M2, glutathione S-alkyltransferase M2, glutathione S-aralkyltransferase M2, glutathione S-aryltransferase M2, glutathione S-transferase 4, glutathione S-transferase M1, glutathione S-transferase M2 (muscle), glutathione S-transferase mu 2
Official Symbol: GSTM1 / GSTM2
Accession Number(s): NP_000552.2; NP_666533.1; NP_000839.1
Human GeneID(s): 2944 , 2946
Important Comments: This antibody is expected to recognise both reported variants of human GSTM1 protein (NP_000552.2; NP_666533.1) and wil also recognise human GSTM2 protein (NP_000839.1).

Immunogen

Peptide with sequence CVFSKMAVWGNK, from the C Terminus of the protein sequence according to NP_000552.2; NP_666533.1; NP_000839.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 26kDa band observed in Human Liver and Human Lung lysates (calculated MW of 25.7kDa according to NP_000552.2, and NP_000839.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following paper:
Endo K, Uno S, Seki T, Ariga T, Kusumi Y, Mitsumata M, Yamada S, Makishima M.
Inhibition of aryl hydrocarbon receptor transactivation and DNA adduct formation by CYP1 isoform-selective metabolic deactivation of benzo[a]pyrene.
Toxicol Appl Pharmacol. 2008 Jul 15;230(2):135-43. Epub 2008 Feb 21.
PMID: 18372000

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