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Goat Anti-AKR1C3 Antibody (EB06637)

Code Name Applications Availability Size Tested species Price Grade  
EB06637 Goat Anti-AKR1C3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB06637 1
EB06637 (0.03µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06637 2
EB06637 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKR1C3, DD3, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), prostaglandin F synthase, dihydrodiol dehydrogenase 3, chlordecone reductase homolog, hydroxysteroid (17-beta) dehydrogenase 5, type IIb 3-alpha hydroxysteroid dehydrogenase, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, DDX, aldo-keto reductase family 1, member C3, chlordecone reductase, dihydrodiol dehydrogenase X, type II 3a-hydroxysteroid dehydrogenase, type IIb 3-alpha hydroxysteroid dehydrogenase
Official Symbol: AKR1C3
Accession Number(s): NP_003730.4
Human GeneID(s): 8644

Immunogen

Peptide with sequence CFASHPNYPYSDEY, from the C Terminus of the protein sequence according to NP_003730.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in Human Liver and Human Breast lysates (calculated MW of 36.9kDa according to NP_003730). Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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