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Goat Anti-SRD5A2 / 5-alpha reductase 2 Antibody (EB06639)

Code Name Applications Availability Size Tested species Price Grade  
EB06639 Goat Anti-SRD5A2 / 5-alpha reductase 2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB06639 1
EB06639 (0.3µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SRD5A2, steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2), steroid 5-alpha-reductase 2, MGC138457, 3-oxo-5 alpha-steroid 4-dehydrogenase 2
Official Symbol: SRD5A2
Accession Number(s): NP_000339.2
Human GeneID(s): 6716

Immunogen

Peptide with sequence QVQCQQSPVLAG, from the N Terminus of the protein sequence according to NP_000339.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 26kDa band observed in Human Prostate and Breast lysates (calculated MW of 28.4kDa according to NP_000339.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human