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Goat Anti-Proopiomelanocortin / POMC Antibody (EB06727)

Related Products: EBP06727 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06727 Goat Anti-Proopiomelanocortin / POMC Antibody Pep-ELISA, IHC, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB06727 1
EB06727 (2µg/ml) staining of paraffin embedded Human Pituitary Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
EB06727 2
EB06727 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin, LPH, NPP, OTTHUMP00000119991, OTTHUMP00000200964, adrenocorticotropic hormone, adrenocorticotropin, alpha-melanocyte-stimulating hormone, beta-LPH, beta-MSH, beta-melanocyte-stimulating hormone, corticotropin-like intermediary peptide, gamma-LPH, gamma-MSH, lipotropin beta, lipotropin gamma, melanotropin alpha, melanotropin beta, melanotropin gamma, met-enkephalin, pro-ACTH-endorphin, proopiomelanocortin preproprotein
Official Symbol: POMC
Accession Number(s): NP_000930.1; NP_001030333.1
Human GeneID(s): 5443

Immunogen

Peptide with sequence C-NAIIKNAYKKGE, from the C Terminus of the protein sequence according to NP_000930.1; NP_001030333.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary testing showed a band at approx 30kDa in A431 cell lysate after 1µg/ml antibody staining (calculated MW of 29.4kDa according to NP_000930.1). Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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