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Goat Anti-RICTOR Antibody (EB06732)

Related Products: EBP06732 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06732 Goat Anti-RICTOR Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06732 1
EB06732 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: Rictor, KIAA1999, AVO3, MGC39830, likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae), rapamycin-insensitive companion of mTOR, mAVO3, pianissimo, DKFZp686B11164, RICTOR, RPTOR independent companion of MTOR, complex 2, PIA, mAVO3, TORC2-specific protein AVO3
Official Symbol: RICTOR
Accession Number(s): NP_689969.2
Human GeneID(s): 253260
Non-Human GeneID(s): 78757 (mouse)

Immunogen

Peptide with sequence C-KQPIVDTSAES, from the C Terminus of the protein sequence according to NP_689969.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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