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Goat Anti-Renalase (aa 224 to 233) Antibody (EB06747)

Related Products: EBP06747 - Immunizing Peptide, EBP07855 - Immunizing Peptide, Goat Anti-Renalase (aa 134 to 147) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06747 Goat Anti-Renalase (aa 224 to 233) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc
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EB06747 1
EB06747 (1µg/ml) staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: renalase, C10orf59, chromosome 10 open reading frame 59, HGNC:25641, FLJ11218, hypothetical protein LOC55328, RNLS, renalase, FAD-dependent amine oxidase, RENALASE
Official Symbol: RNLS
Accession Number(s): NP_001026879.2; NP_060833.1
Human GeneID(s): 55328
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001026879.2 and NP_060833.1).

Immunogen

Peptide with sequence C-VSIDNKKRNI, from the internal region of the protein sequence according to NP_001026879.2; NP_060833.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38+35kDa bands observed in Human Heart lysates, 37kDa band observed in Mouse Heart lysates and approx 35kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3kDa according to Mouse NP_001161290.1 and of 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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