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Goat Anti-Collagen, type VII Antibody (EB06781)

Related Products: EBP06781 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06781 Goat Anti-Collagen, type VII Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06781 1
EB06781 (0.5µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Collagen, type VII, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), COL7A1, HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen, collagen, type VII, alpha 1
Official Symbol: COL7A1
Accession Number(s): NP_000085.1
Human GeneID(s): 1294
Non-Human GeneID(s): 12836 (mouse)
Important Comments: This product does not work on cryostat sections of Normal Human Skin.

Immunogen

Peptide with sequence C-TVQYSDDPRTEF, from the internal region of the protein sequence according to NP_000085.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 250kDa band observed in Rat Testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5µg/ml.

A customer found this antibody unsuccessful on cryosections of Human Skin.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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