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Goat Anti-AKT1 Antibody (EB06875)

Related Products: EBP06875 - Immunizing Peptide, EBP07691 - Immunizing Peptide, Goat Anti-AKT1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06875 Goat Anti-AKT1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06875 1
EB06875 (2µg/ml) staining of MCF7 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: AKT1, v-akt murine thymoma viral oncogene homolog 1, HGNC:391, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha
Official Symbol: AKT1
Accession Number(s): NP_005154.2; NP_001014432.1; NP_001014431.1
Human GeneID(s): 207
Important Comments: Variants one, two and three encode the same protein (NP_005154, NP_001014432, NP_001014431). No cross-reactivity expected with AKT2 or AKT3.

Immunogen

Peptide with sequence C-EVSLAKPKHR, from the internal region of the protein sequence according to NP_005154.2; NP_001014432.1; NP_001014431.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in MCF7 and approx. 60-65kDa in NIH3T3 cell lysates (calculated MW of 55.7kDa according to Human NP_005154.2 and Mouse NP_033782.1). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3ug/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

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