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Goat Anti-GDF15 Antibody (EB06909)

Related Products: EBP06909 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06909 Goat Anti-GDF15 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06909 1
EB06909 (0.5µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06909 2
EB06909 (0.1ug/ml) staining of A549 lysate 1 (A), CaCo-2 lysate 1 (B) and (0.3ug/ml) LNCaP lysate 1 (C) (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06909 3
EB06909 (0.3ug/ml) staining of A549 lysate 2 (A) + peptide (B), CaCo-2 lysate 2 (C) + peptide (D), LNCaP lysate 2 (E) + peptide (F) (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06909 4
EB06909 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06909 5
EB06909 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06909 6
EB06909 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
Official Symbol: GDF15
Accession Number(s): NP_004855.2
Human GeneID(s): 9518

Immunogen

Peptide with sequence C-QKTDTGVSLQTYDD, from the C Terminus of the protein sequence according to NP_004855.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Human Prostate lysates and in lysates of cell lines A549, CaCo-2 and LNCaP (calculated MW of 34.1kDa according to NP_004855.2). An additional band was seen at 48kDa in some lysates and was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.5ug/ul. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 7µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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