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Goat Anti-BRSK2 / STK29 Antibody (EB06981)

Related Products: EBP06981 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06981 Goat Anti-BRSK2 / STK29 Antibody Pep-ELISA, FC, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06981 1
EB06981 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER/Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06981 2
EB06981 Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: BRSK2, SAD1, STK29, PEN11B, C11orf7, BR serine/threonine kinase 2, serine/threonine kinase 29, homolog of SAD-1 (C. elegans), chromosome 11 open reading frame 7, chromsosome 11 open reading frame 7, FLJ41362, protein kinase SAD1B, serine/threonine kinase SAD-A
Official Symbol: BRSK2
Accession Number(s): NP_003948.2; NP_001243556.1; NP_001243558.1; NP_001243559.1; NP_001269147.1
Human GeneID(s): 9024
Non-Human GeneID(s): 75770 (mouse), 293631 (rat)

Immunogen

Peptide with sequence CPEVIRGEKYDGRKAD, from the internal region of the protein sequence according to NP_003948.2; NP_001243556.1; NP_001243558.1; NP_001243559.1; NP_001269147.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.

Immunofluorescence: Strong expression of the protein seen in the ER/Golgi and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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