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Code Name Applications Availability Size Tested species Price Grade  
EB07013 Goat Anti-SH2D1A/SLAM associated protein Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07013 1
EB07013 (0.2µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07013 2
HEK293 overexpressing SH2D1A (RC204723) and probed with EB07013 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: SH2D1A, SH2 domain protein 1A, Duncan\1s disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, SAP, XLP, XLPD, SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, T cell signal transduction molecule SAP, RP5-1052M9.3, FLJ18687, FLJ92177, Duncan's disease (lymphoproliferative syndrome), Duncan's disease, SLAM-associated protein, T cell signal transduction molecule SAP; signaling lymphocyte activation molecule-associated protein
Official Symbol: SH2D1A
Accession Number(s): NP_002342.1; NP_001108409.1
Human GeneID(s): 4068

Immunogen

Peptide with sequence C-QYPVEKKSSARSTQ, from the internal region of the protein sequence according to NP_002342.1; NP_001108409.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.2kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx. 17kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6µg/ml.

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig,

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