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Goat Anti-FZD8 / frizzled 8 Antibody (EB07028)

Related Products: EBP07028 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07028 Goat Anti-FZD8 / frizzled 8 Antibody Pep-ELISA, WB, IHC, IF, EM Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07028 1
EB07028 (1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07028 2
EB07028 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-
Click here to download PDF datasheet.

Target Protein

Principal Names: FZD8, frizzled homolog 8 (Drosophila), HGNC:4046, FZ-8, hFZ8, frizzled (Drosophila) homolog 8, frizzled 8
Official Symbol: FZD8
Accession Number(s): NP_114072.1
Human GeneID(s): 8325
Non-Human GeneID(s): 14370 (mouse)

Immunogen

Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in Human Pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B et al. (2019) PMID: 21321602.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 3µg/ml.

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.

Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining): Zhao B et al. (2019) PMID: 21321602.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in WB, IF & EM on Mouse:
Zhao B, Wang HB, Lu YJ, Hu JW, Bao L, Zhang X.
Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretoryvesicles.
Cell Res. 2011 May;21(5):741-53.
PMID: 21321602

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