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Code Name Applications Availability Size Tested species Price Grade  
EB07041 Goat Anti-PIM2 (Internal) Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07041 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with EB07041 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB07041 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PIM2, pim-2 oncogene, HGNC:8987, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2
Official Symbol: PIM2
Accession Number(s): NP_006866.2
Human GeneID(s): 11040

Immunogen

Peptide with sequence C-PPGGKDREAFEAE, from the internal region of the protein sequence according to NP_006866.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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