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Goat Anti-Adenosine A2a Receptor Antibody (EB07079)

Related Products: EBP07079 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07079 Goat Anti-Adenosine A2a Receptor Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse, Macaque $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB07079 1
EB07079 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ADORA2A, adenosine A2a receptor, HGNC:263, ADORA2, RDC8, hA2aR, adenosine A2 receptor, adenosine receptor subtype A2a
Official Symbol: ADORA2A
Accession Number(s): NP_000666.2
Human GeneID(s): 135

Immunogen

Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: This antibody has been successfully used in Western blot on Macaque: van der Putten C et al. (2009) PMID: 19494284. This antibody has been successfully used in Western blot on Mouse: He X et al. (2013) PMID: 23712033, and Zhao L et al. (2015) PMID: 26171727.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Macaque
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in WB on Mouse:
Zhao L, Liu YW, Yang T, Gan L, Yang N, Dai SS, He F.
The mutual regulation between miR-214 and A2AR signaling plays an important role in inflammatory response.
Cell Signal. 2015 Oct;27(10):2026-34.
PMID: 26171727

This antibody has been successfully used in the WB on Mouse:
He X, Hu JL, Li J, Zhao L, Zhang Y, Zeng YJ, Dai SS, He FT.
A feedback loop in PPARγ-adenosine A2A receptor signaling inhibits inflammation and attenuates lung damages in a mouse model of LPS-induced acute lung injury.
Cell Signal. 2013 Sep;25(9):1913-23.
PMID: 23712033

This antibody has been successfully used in WB on Macaque:
van der Putten C, Zuiderwijk-Sick EA, van Straalen L, de Geus ED, Boven LA, Kondova I, IJzerman AP, Bajramovic JJ.
Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia.
J Immunol. 2009 Jun 15;182(12):7603-12.
PMID: 19494284

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