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Goat Anti-EIF2C1 / AGO1 Antibody (EB07129)

Related Products: EBP07129 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07129 Goat Anti-EIF2C1 / AGO1 Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07129 1
HEK293 overexpressing EIF2C1 and probed with EB07129 (non-transfected HEK293 in lane B).
EB07129 2
EB07129 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07129 3
EB07129 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EIF2C1, eukaryotic translation initiation factor 2C, 1, AGO1, EIF2C, GERP95, Q99, Golgi Endoplasmic Reticulum protein 95 kDa, argonaute 1, putative RNA-binding protein Q99
Official Symbol: AGO1
Accession Number(s): NP_036331.1
Human GeneID(s): 26523
Important Comments: This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.

Immunogen

Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2kDa according to NP_036331.1. Recommended concentration, 0.3-1µg/ml.
IHC: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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