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Goat Anti-CDX2 Antibody (EB07190)

Related Products: EBP07190 - Immunizing Peptide, Goat Anti-CDX2 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07190 Goat Anti-CDX2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07190 1
EB07190 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07190 2
EB07190 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDX2, caudal type homeo box transcription factor 2, HGNC:1806, CDX-3, CDX3
Official Symbol: CDX2
Accession Number(s): NP_001256.1
Human GeneID(s): 1045
Non-Human GeneID(s): 12591 (mouse), 66019 (rat)
Important Comments: No cross-reactivity expected with CDX1 and 4

Immunogen

Peptide with sequence EQLSPGGQRRNLCE, from the internal region of the protein sequence according to NP_001256.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Colon. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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