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Goat Anti-CYP3A4 Antibody (EB07194)

Related Products: EBP07194 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07194 Goat Anti-CYP3A4 Antibody Pep-ELISA, IHC, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
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EB07194 1
EB07194 (5µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07194 2
EB07194 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
EB07194 3
EB07194 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4, HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, cytochrome P450, subfamily IIIA, polypeptide 4, glucocorticoid-inducible P450, nifedipine oxidase
Official Symbol: CYP3A4
Accession Number(s): NP_059488.2; NP_001189784.1
Human GeneID(s): 1576
Important Comments: This antibody is expected to recognise both reported isoforms (NP_059488.2; NP_001189784.1) and it is not expected to cross-react with 3A5, 3A7 ot 3A43.

Immunogen

Peptide with sequence C-KESRLEDTQKHR, from the internal region of the protein sequence according to NP_059488.2; NP_001189784.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5-7µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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