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Goat Anti-SMAD9 Antibody (EB07200)

Related Products: EBP07200 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07200 Goat Anti-SMAD9 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07200 1
EB07200 (0.3µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07200 2
EB07200 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)], HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B, MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, MAD, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
Official Symbol: SMAD9
Accession Number(s): NP_005896.1
Human GeneID(s): 4093
Important Comments: This antibody is not expected to cross-react with SMAD1 and SMAD5.

Immunogen

Peptide with sequence C-HSEPLMPHNATYPD, from the internal region of the protein sequence according to NP_005896.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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