Choose your country here (United States of America)
Home

Goat Anti-SOD1, Biotinylated Antibody (EB07208-B)

Code Name Applications Availability Size Tested species Price Grade  
EB07208-B Goat Anti-SOD1, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $423.00 Elite

Ordering Information for United States of America (change)

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

EB07208-B 1
Biotinylated EB07208 (0.3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: SOD1, superoxide dismutase 1, soluble, ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, Cu/Zn superoxide dismutase, SOD, soluble, epididymis secretory protein Li 44, indophenoloxidase A, superoxide dismutase, cystolic, HGNC:11179
Official Symbol: SOD1
Accession Number(s): NP_000445.1
Human GeneID(s): 6647
Non-Human GeneID(s): 20655 (mouse), 24786 (rat)

Immunogen

Peptide with sequence C-SRKHGGPKDEERH., from the internal region of the protein sequence according to NP_000445.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 20kDa band observed in ysates of cell line HEK293 (calculated MW of 15.9kDa according to NP_000445.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human