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Goat Anti-HRS / HGS Antibody (EB07211)

Related Products: EBP07211 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07211 Goat Anti-HRS / HGS Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07211 1
EB07211 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HGS, hepatocyte growth factor-regulated tyrosine kinase substrate, HGNC:4897, HRS, ZFYVE8, human growth factor-regulated tyrosine kinase substrate
Official Symbol: HGS
Accession Number(s): NP_004703.1
Human GeneID(s): 9146
Non-Human GeneID(s): 15239 (mouse), 56084 (rat)

Immunogen

Peptide with sequence C-NRNYWEKKQEEARK, from the internal region of the protein sequence according to NP_004703.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: This antibody has been successfully used in Western Blot on Human: Clancy et al. (2020) https://doi.org/10.1101/2020.04.15.042291.
IHC: In paraffin embedded Human Testis shows strong staining of Leydig cells. Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western Blot on Human:
Clancy et al
The deubiquitylase USP9X controls ribosomal stalling
J Cell Biol. 2021 Mar 1;220(3):e202004211
PMID: 33507233

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