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Goat Anti-Casein Kinase 1, delta Antibody (EB07232)

Related Products: EBP07232 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07232 Goat Anti-Casein Kinase 1, delta Antibody Pep-ELISA, WB, IF Allow up to 10 days for delivery 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07232 1
EB07232 (0.2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07232 2
EB07232 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: casein kinase 1, delta, HGNC:2452, CSNK1D, HCKID, CKIdelta
Official Symbol: CSNK1D
Accession Number(s): NP_001884.2; NP_620693.1
Human GeneID(s): 1453
Non-Human GeneID(s): 104318 (mouse), 64462 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001884.2 and NP_620693.1)

Immunogen

Peptide with sequence C-DREERLRHSRNPATR, from the internal region of the protein sequence according to NP_001884.2; NP_620693.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in Mouse Brain lysates and in preliminary testing of U251 cell lysates (calculated MW of 47.3kDa according to Mouse NP_620690.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Expression of the protein seen in the Nucleus of U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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