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Goat Anti-ACSL5 Antibody (EB07250)

Related Products: EBP07250 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07250 Goat Anti-ACSL5 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07250 1
EB07250 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07250 2
EB07250 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACSL5, acyl-CoA synthetase long-chain family member 5, HGNC:16526, ACS2, ACS5, FACL5, fatty-acid-Coenzyme A ligase, long-chain 5, long-chain acyl-CoA synthetase 5, long-chain fatty acid coenzyme A ligase 5
Official Symbol: ACSL5
Accession Number(s): NP_057318.2; NP_976313.1; NP_976314.1
Human GeneID(s): 51703
Important Comments: This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).

Immunogen

Peptide with sequence C-RTQIDSLYEHIQD, from the C Terminus of the protein sequence according to NP_057318.2; NP_976313.1; NP_976314.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in Human Spleen lysates (calculated MW of 76.0kDa according to NP_976313.1and NP_976314.1). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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