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Goat Anti-EBAG9 / RCAS1 Antibody (EB07292)

Related Products: EBP07292 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07292 Goat Anti-EBAG9 / RCAS1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07292 1
EB07292 (0.2µg/ml) staining of A431 lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
EB07292 2
EB07292 (3.75µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EBAG9, RCAS1, estrogen receptor binding site associated, antigen, 9, HGNC:3123, EB9, PDAF, cancer associated surface antigen, estrogen receptor binding site associated antigen 9
Official Symbol: EBAG9
Accession Number(s): NP_004206.1
Human GeneID(s): 9166
Non-Human GeneID(s): 55960 (mouse), 299864 (rat)

Immunogen

Peptide with sequence C-KEAQRLMKKEQN, from the C Terminus of the protein sequence according to NP_004206.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 26kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 24.4kDa according to NP_004206.1and NP_004206.1). Recommended concentration: 1-3µg/ml. A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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